Conference Proceeding

Nitrogen-deficiency Stress Reveals Differentially Expressed Proteins in Low-N Tolerant and Low-N Sensitive Maize Genotypes

Dr. Muslima Nazir

Nitrogen (N) is a critical element for plant growth and its application has proven to be a valuable agricultural practice. However, unavoidable economic and environmental problems associated with excessive use of N-fertilizers demands its better management. The solution lies in improving the N use efficiency to sustain productivity even at low N levels. Evaluation of 32 maize genotypes using physiological and biochemical traits under optimum (4.5 mM) and low N (0.5 mM) was carried out. The contrasting low-N sensitive (HM-4) and low-N tolerant (PEHM-2) genotypes were used for comparative proteomeprofiling of leaves under optimum and low N as well as restoration of low N on 3rd (NR3) and 5th (NR5) days after resupplying N. The analysis of differential expression pattern of proteins was performed by 2-D gel electrophoresis. Significant variations in the expression of proteins were observed under low N, which were genotype specific. In the leaf proteome, 25 spots were influenced by N treatment and 4 spots were different between the two genotypes. Most of the proteins that were differentially accumulated in response to N level and were involved in photosynthesis and metabolism, affirming the relationship between N and carbon metabolism. In addition to this, greater intensity of some defense proteins in the low N tolerant genotype was found that may have a possible role in imparting it tolerance under N starvation conditions. The new insights generated on maize proteome in response to N-starvation and restoration would be useful towards improvement of N-use efficiency in maize.

Published: 08 November 2017


Copyright: © 2017 Dr. Muslima Nazir. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.